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Acute, prolonged, and chronic exposure to organochlorine insecticides evoke differential effects on in vitro neuronal activity and network development
Our latest study from the ENDpoiNTs project has been published in NeuroToxicology!
In this study, we examined the effects of acute (30 minutes), prolonged (up to 48 h), and chronic (21 days) exposure to DDT, its metabolite DDE, endosulfan, dieldrin, and lindane on neuronal activity and network development in sex-separated rat primary cortical cultures using micro-electrode array (MEA) recordings. Our study showed that acute exposure to all tested organochlorines evoked a hyperexcitation, sometimes at concentrations ≤1 µM. Exposure to DDT, DDE, endosulfan, and dieldrin inhibited neuronal activity after prolonged exposure, while exposure to lindane had no clear effects after 24 and 48 hours. Chronic exposure to all tested organochlorines inhibited neuronal network development at high micromolar concentrations. Most of these effects were observed at non-cytotoxic concentrations, except for exposure to 100 µM endosulfan. Some of the effects found in this study differed between male and female cultures, but no clear sex-specific patterns could be determined across exposure scenarios or between compounds. Together, these results show that acute, prolonged, and chronic exposure to organochlorine insecticides evoke differential effects on neuronal activity and network development. The effects found after acute exposure to DDT, endosulfan, dieldrin, and lindane occur at concentrations close to, or even below human internal exposure levels, highlighting the importance of further monitoring human exposure to organochlorine insecticides.
For more details see the full article (open access) here:
